Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody

doi:10.7506/spkx1002-6300-200921050

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21 ): 214-216.doi: 10.7506/spkx1002-6300-200921050

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Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody

TONG Ping1,2，GAO Jin-yan3，GUO Xiao-ying4，CHEN Hong-bing1,2,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；2. Sino-German Joint
Research Institute, Nanchang University, Nanchang 330047, China；3. Department of Food Science, Nanchang University,
Nanchang 330047, China；4. Nanchang Agri-food and Safety Inspection Center, Nanchang 330009, China

Received:2009-07-12 Online:2009-11-01 Published:2010-12-29
Contact: CHEN Hong-bing E-mail:chbgjy@hotmail.com

Abstract

Abstract:

In order to prepare highly specific rabbit anti-ovotransferrin polyclonal antibody, New Zealand rabbits were immunized with commercial ovotransferrin or laboratory-purified ovotransferrin. The titer and specificity of antisera prepared were analyzed by ELISA and Western blotting, respectively. Results showed that all the antisera reached a titer of 1:819200 and exhibited a high specificity. Therefore, target products have been successfully prepared, which might be useful for the further investigations of egg allergy.

Key words: ovotransferrin (OVT), polyclonal antibody, egg allergy

CLC Number:

Q813.2

TONG Ping1,2，GAO Jin-yan3，GUO Xiao-ying4，CHEN Hong-bing1,2,*. Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody[J]. FOOD SCIENCE, 2009, 30(21 ): 214-216.

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Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody

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