FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21): 241-245.doi: 10.7506/spkx1002-6300-200921057

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A High Cholesterol-reducing Lactic Acid Bacterial Strain: Screening, Identification and Probiotic Potential Evaluation

ZENG Xiao-qun1 PAN Dao-dong1,2,* YANG Yao1 GUO Yu-xing1 LIU Ying-ting1   

  1. 1.Branch Center of National Dairy Products Processing Technology Development, Nanjing Normal University, Nanjing 210097,
    China 2.College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
  • Received:2008-12-31 Revised:2009-07-29 Online:2009-11-01 Published:2010-12-29
  • Contact: PAN Dao-dong E-mail:daodongpan@163.com

Abstract:

A probiotic strain with an excellent cholesterol-reducing ability of 50.83 % was screened from 5 strains of lactic acid bacteria, which were isolated from fresh cattle feces. This strain was identified as Lactobacillus fermentum with API identification system and 16srRNA sequence alignment and was nominated as Lactobacillus fermentum DF-4. It exhibited a strong tolerance to 0.3% bile salt and low pH and an antagonistic effect against both enteropathogenic E.coli and S.aureas when incubated in the simulated gastroenteric environment. These results indicate that Lactobacillus fermentum DF-4 is a potential probiotics with an excellent cholesterol-reducing effect.

Key words: screening, identification, Lactobacillus fermentum DF-4 , cholesterol-reducing effect , probiotics

CLC Number: