Breeding of A Novel Pleurotus citrinopileatus Strain

doi:10.7506/spkx1002-6300-200921069

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21): 301-304.doi: 10.7506/spkx1002-6300-200921069

Previous Articles Next Articles

Breeding of A Novel Pleurotus citrinopileatus Strain

CAI De-hua1，ZHANG Qiu-sheng1,*，DONG Hong-xin1，WANG Xiang-lan2，LIU Shu-fang3，WANG Yan-jie1

1. College of Life Science, Ludong University, Yantai 264025, China ；2. First Middle School of Haiyang, Haiyang 265100,
China；3. Teacher Training School of Haiyang, Haiyang 265100, China

Received:2008-11-07 Revised:2009-08-03 Online:2009-11-01 Published:2010-12-29
Contact: ZHANG Qiu-sheng E-mail:qiushengzhang@126.com

Abstract

Abstract:

The effect of 60Co-γ irradiation with different doses and dose rates on mycelial growth of Pleurotus citrinopileatus was investigated in this study. Mutant strains of Pleurotus citrinopileatus with high growth rate were obtained through 60Co-γ irradiation at 600 Gy and 43.5 Gy/h dose rate. After the regeneration of mycelia, a novel strain having the highest growth rate was screened, which showed an average increase of 21.1% when compared with that of the parental strain. Results of antagonistic test, esterase isozyme and peroxidase isozyme electrophoresis revealed significant genetic differences between the mutant strain and its parent stain. After six generations of continuous cultivation, the mutant strain kept the good growth vigor, thereby displaying high genetic stability.

Key words: Pleurotus citrinopileatus, mutagenesis, breeding

CLC Number:

S646

CAI De-hua1，ZHANG Qiu-sheng1,*，DONG Hong-xin1，WANG Xiang-lan2，LIU Shu-fang3，WANG Yan-jie1. Breeding of A Novel Pleurotus citrinopileatus Strain[J]. FOOD SCIENCE, 2009, 30(21): 301-304.

[1]	GAO Zhaojian, WANG Qiufen, DING Feihong, XU Xiang, ZHAO Yifeng, JIAO Wei, CHEN Teng. Screening and Mutagenesis of Broad-Spectrum Antagonistic Bacillus licheniformis and Purification and Identification of Antimicrobial Substances Produced by Its Mutant [J]. FOOD SCIENCE, 2021, 42(2): 143-150.
[2]	LI Xiaohan, GUO Jiaomei, SONG Kai, SUN Jingjing, WANG Wei, HAO Jianhua,. Improvement of the Product Specificity of Bacillus sp. Y112 Cyclodextrin Glucosyltransferase by Site-Directed Mutagenesis of Arginine 81 [J]. FOOD SCIENCE, 2021, 42(10): 133-138.
[3]	LÜ Bianmei, JIANG Xuewei, PENG Dong, XU Xiaogang, DING Yuan, LUO Xiaoming, ZHOU Shangting. Breeding and Application of Salt-Tolerant Ethanol-Producing Yeast for Soy Sauce Brewing [J]. FOOD SCIENCE, 2020, 41(20): 119-126.
[4]	LIU Le, DING Yi, WANG Zixuan, FANG Yaowei, WANG Shujun, Lü Mingsheng. Dextranase from Arthrobacter oxydans KQ11: Identification of Key Residues in Catalysis [J]. FOOD SCIENCE, 2019, 40(6): 113-120.
[5]	ZHAO Tianhui, ZHANG Baiqing. Pulsed Light Mutagenesis and Screening of Bacillus subtilis for Improved Tolerance and Extracellular Enzymatic Activities of Screened Mutants [J]. FOOD SCIENCE, 2018, 39(2): 192-197.
[6]	LIU Zongmin, TAN Xinghe, ZHOU Hongli, WANG Feng, GUO Hongying, YAO He1,LIU Chucen, WANG Lanshu, YAN Qinwu, XU Yongbing. Quality Changes of Dried Radish during Fermentation by Lactobacillus plantarum [J]. FOOD SCIENCE, 2018, 39(2): 198-202.
[7]	YUAN Lin, ZENG Jing, GUO Jianjun, GUO Hao, YANG Gang, CHEN Jun. Efficient Secretory Expression of Hyperthermoacidophilic α-Amylase PFA in Bacillus subtilis WB600 [J]. FOOD SCIENCE, 2018, 39(18): 100-108.
[8]	LI Wen, DONG Mingsheng. Improving γ-Aminobutyric Acid Production of Lactic Acid Bacteria in Chickpea Milk by Compound Mutagenesis [J]. FOOD SCIENCE, 2018, 39(16): 147-153.
[9]	MA Jiage, YU Wei, LI Jiajun, ZHOU Shihao. Antibacterial Mechanism of Bacteriocin from Lactobacillus casei [J]. FOOD SCIENCE, 2018, 39(10): 193-198.
[10]	ZHAO Xiuli, ZHANG Shan, SONG Lu, WANG Meng, HOU Lihua. Construction of Uracil Auxotrophic Strains of Salt Tolerant Yeasts [J]. FOOD SCIENCE, 2017, 38(8): 11-16.
[11]	WANG Tao, CHU Yuanming, CHEN Hongwei, LI Wen, GAO Mingxia, DONG Yuwei, LI Tongxiang, ZHANG Chuanli. Zinc Enrichment of Cordyceps militaris Cultured in Liquid Medium: Optimization of Medium Components and Culture Conditions and Strain Improvement by LiCl Mutagenesis [J]. FOOD SCIENCE, 2017, 38(6): 74-80.
[12]	ZENG Jing, GUO Jianjun, YUAN Lin. Improvement of the Thermal Activity and Stability of Hyperthermophilic α-Amylase ApkA by Site-Directed Mutagenesis [J]. FOOD SCIENCE, 2017, 38(2): 20-26.
[13]	MA Qing, CAI Rui, JIANG Fengchao, MA Lijuan, DU Liping, XIAO Dongguang. Effect of N-Glycosylation on the Heterologous Expression of β-Mannanase in Pichia pastoris [J]. FOOD SCIENCE, 2017, 38(16): 86-91.
[14]	XU Yan, GUO Qian, ZHU Yibo, WANG Limei, QI Bin. Screening of Phenyllactic Acid-Resistant Escherichia coli and Its Application in the Synthesis of Phenyllactic Acid [J]. FOOD SCIENCE, 2017, 38(14): 24-29.
[15]	JIA Rui, ZHANG Chong, Lü Fengxia, BIE Xiaomei, ZHAO Haizhen, LU Zhaoxin. Breeding and Comparative Analysis of High-Yield Bacitracin-Producing Bacillus licheniformis by Genome Shuffling [J]. FOOD SCIENCE, 2016, 37(23): 135-140.

Breeding of A Novel Pleurotus citrinopileatus Strain

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments