Abstract:

In the present study, grape seed oil extraction technique with the assistance of cellulase or ultrasonic was optimized using orthogonal array design. In addition to this, grape seed oil samples obtained using both techniques were also evaluated for physico-chemical properties. Results showed that the optimum values of technological parameters were hydrolysis temperature 50 ℃, hydrolysis time 1.5 h, pH 5.5, and enzyme dose 300 U/g for cellulase-assisted extraction, and ultrasound power 500 W, temperature 30 ℃, treatment 30 min and solid/liquid ratio 1:12 (g/m1) for ultrasonic-assisted extraction, respectively. Ultrasonic-assisted extraction gave higher oil yield and had the advantages of time saving and low cost when compared with cellulase-assisted extraction. However, lower peroxidation, acid and iodine values and moisture and volatile matter contents, higher refractive index and slightly different relative density were observed in grape seed oil obtained using cellulase-assisted extraction in comparison with using ultrasonic-assisted extraction. Therefore, from the viewpoint of product quality, cellulaseassisted extraction is superior to ultrasonic-assisted extraction.

Key words: grape seed oil, cellulase, ultrasonic, physico-chemical properties