Abstract:

Pancreatin was extracted from pig pancreas using isopropyl alcohol as solvent and chitosan as precipitant. The effect of isopropyl alcohol concentration, pH, extraction time and amount of chitosan on the yield and activities of pancreatin was stuided by conducting single factor and the following orthogonal test. The optimum extraction conditions were that pig pancreas was extracted with 7.5% isopropyl at pH 4 for 6 h, and pancreatin was precipitated with 0.1% chitosan. Under such conditions, the yield of pancreatin was 12.19%, and the activities of trypsin, amylopsin and lipase in it was 4836, 14320, 20580 U/g respectively. Chitosan, as a new precipitan, was effective in the extraction of pancreatin.

Key words: pig pancreas, chitosan, pancreatin, extraction