FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 250-252.doi: 10.7506/spkx1002-6300-200922057

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Use of SDS-PAGE Electrophoresis for Separation and Determination of Fibrinogen in Bovine Serum

LIU Ai-guo,WU Zi-jian,GUAN Mei-ling,JIN Bei-bei,HU Jing,YANG Gui-you   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,
    Tianjin 300134, China
  • Received:2009-07-03 Online:2009-11-15 Published:2010-12-29
  • Contact: LIU Ai-guo, E-mail:liuaiguo@tjcu.edu.cn

Abstract:

Fibrinogen in bovine serum was determined using tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, and the optimum condition were obtained as follows: separation gel concentration 12.5%, cross linking degree 3% and the concentration of spacer gel 5%. Fibrinogen was separated from bovine serum using 21% ammonium sulfate precipitation at 4 ℃, and the fibrinogen residue in bovine serum was reduced to 0.02473%.

Key words: bovine serum, fibrinogen, determination, separation

CLC Number: