Abstract:

Fibrinogen in bovine serum was determined using tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, and the optimum condition were obtained as follows: separation gel concentration 12.5%, cross linking degree 3% and the concentration of spacer gel 5%. Fibrinogen was separated from bovine serum using 21% ammonium sulfate precipitation at 4 ℃, and the fibrinogen residue in bovine serum was reduced to 0.02473%.

Key words: bovine serum, fibrinogen, determination, separation