FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22): 290-292.doi: 10.7506/spkx1002-6300-200922068

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HPLC Determination of 2-Hydroxybiphenyl in Fermentation Broth of Desulfurization Strain

GE Jing-ping,WANG Li-li,PING Wen-xiang*   

  1. Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080,China
  • Received:2008-11-28 Online:2009-11-15 Published:2010-12-29
  • Contact: PING Wen-xiang E-mail:wenxiangp@yahoo.com.cn

Abstract:

The concentration of 2-hydroxybiphenyl (2-HBP) in the fermentation broth of desulfurization strain was determined using HPLC. 2-HBP was separated thourgh a ODS column (3.9×150 mm, 5 μm, Delta-PakTM, Waters), eluted with 70% aqueous methanol at 1 ml/min, and detected at 260 nm. Results showed that retention time of 2-HBP was 2.6 min with the detection limits 0~80 mg/L and the 2-HBP concentration in the fermentation broth of strain HDBRS-1 was 6.11 mg/L.

Key words: HPLC, 2-HBP, detection

CLC Number: