Abstract:

In order to investigate the antioxidant function of black soybean peptide (BSP) in D-galactose-induced senescent mice, fifty Kunming mice were divided into five groups, designated as control group, oxidative injury model group, VC positive control group, and BSP high- and low-dose groups. Except the control group, all other groups were intraperitoneally injected with D-galactose solution at the daily dose of 400 mg/kg bw·d for 56 successive days. At the same time, BSP-treated mice were administered BSP by gavage at the daily dose of 400 mg/kg bw·d and 800 mg/kg bw·d, respectively. Malondialdehyde (MDA) content, glutathione peroxidase (GSH-Px) activity and lipofuscin (LPF) content in serum or in liver were assayed. Moreover, the indexes of spleen, kidney, liver and thymus gland were also determined. Results showed that thymus index, spleen index and MDA content exhibited a significant decrease. While GSH-Px activity revealed a significant decrease both in serum and in liver of oxidative injury mouse model. Both 400 mg/kg bw·d and 800 mg/kg bw·d BSP administrations resulted in a significant increase in thymus index, spleen index and GSH-Px activity in serum or liver and a significant decrease in MDA content. Furthermore, BSP at the dose of 800 mg/kg bw·d also resulted in a significant decrease in LPF content in liver. Therefore, BSP has obvious antioxidant activity in vivo due to its free radical scavenging activity and improved immune organ index for accomplishing anti-aging effect in a dose-dependent mode.

Key words: black soybean peptides (BSP), D-galactose, mice, antioxidant activity