FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 180-183.doi: 10.7506/spkx1002-6300-201005040

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Extraction and Antimicrobial Effect of Active Components from Chaetoceros curvisetus

WANG Qin1,LIU Chao2,ZHANG Shao-jun2,SUN Li-qin2,WANG Chang-hai1,2,*   

  1. (1. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China;
    2. School of Ocean, Yantai University, Yantai 264005, China)
  • Received:2009-04-29 Revised:2009-09-30 Online:2010-03-01 Published:2010-12-29
  • Contact: WANG Chang-hai1 E-mail:chwang2001@sina.com

Abstract:

Antimicrobial activities of crude lipid, polyphenol, terpenoid and water extracts obtained from Chaetoceros curvisetus were evaluated by cylinder plate method. In order to identify the contribution of active components to antimicrobial activity, gas chromatography was used for the characterization of different polar lipid extracts. The minimum inhibitory concentration of petroleum ether extract was also determined. Results indicated that crude lipid, polyphenol and terpenoid extracts exhibited different antimicrobial activities. Petroleum ether extract revealed the strongest antimicrobial activity, and water extract exhibited no antimicrobial activity. All extracts also exhibited stronger inhibitory effect against bacteria than against fungi, especially against gram-negative bacteria. Eight kinds of polyunsatured fatty acids including trans-linoleic acid, γ-linolenic acid, and cis-11,14-eicosadienoic acid were determined in petroleum ether extract, which suggested that the antibacterial components were weakly polar polyunsaturated fatty acids.

Key words: Chaetoceros curvisetus, antimicrobial activities, crude lipid, ployphenol, terpenoid

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