Preliminary Identification and Analysis of Cellulase-producing Strains from Carrot

doi:10.7506/spkx1002-6300-201005052

Abstract

Abstract:

In this study, a strain, HP1, with high cellulase activity was isolated from the surface of carrots. The 16S rDNA fragment of HP1 was identified to be 1435bp by PCR. The sequence was analyzed by bioinformatics method and aligned with sequences published in NCBI Genbank. This strain exhibited 99% similarity with Bacillus cereus. According to colony observation combined with physiological-biochemical analysis, HP1 was preliminarily identified to belong to Bacillus sp., which was a new strain of Bacillus based on sequence and biochemical analyses. The accession number of this strain in NCBI was FJ908092.

Key words: carrot, cellulase, Bacillus, 16S rDNA

CLC Number:

TS201.3

HOU Jin-hui，LIU Quan-de，GAO Ming-xia，GUO Xiang，YE Jun. Preliminary Identification and Analysis of Cellulase-producing Strains from Carrot[J]. FOOD SCIENCE, 2010, 31(5): 233-236 https://doi.org/10.7506/spkx1002-6300-201005052

[1]	YE Wenxin, HE Jiaxin, Vivian Montero ALEJO, YANG Meiyan. Cloning, Expression and Activity Analysis of Four Bacteriophage Lysins [J]. FOOD SCIENCE, 2026, 47(9): 128-137.
[2]	CHEN Haoxuan, LIU Yiping, LIU Xue, ZHOU Wei, YANG Renqin, CHEN Dawei, ZHANG Chenchen, GUAN Chengran. Antibacterial Effects of High-Molecular-Mass Metabolites from Lacticaseibacillus rhamnosus [J]. FOOD SCIENCE, 2026, 47(9): 138-146.
[3]	ZHOU Yingjun, LIU Shimei, SONG Shuai, CAI Jinwen, ZHU Zuohua, YAN Li, PENG Yuande, XIE Chunliang, Yu Na. Enhancing Anti-inflammatory Efficacy and γ-Aminobutyric Acid Content in Enzymatic Hydrolysate of Dendrobium officinale by Fermentation with Lactiplantibacillus plantarum XD117 [J]. FOOD SCIENCE, 2026, 47(8): 174-183.
[4]	YUAN Siqi, HE Qi, ZHAO Jinsong, ZHANG Yadong, YIN Jiebing, LIU Xun, LIU Yufeng, SHEN Yi, LIU Jun. Effect of Mixed Inoculation of Bacillus amyloliquefaciens and Bacillus cereus on the Quality of High-temperature Daqu [J]. FOOD SCIENCE, 2026, 47(7): 143-158.
[5]	TIAN Wenmao, GAO Yue, WANG Ni, LIU Yixue, XU Yu, BI Yang, WANG Yi, LI Yongcai. Postharvest Calcium Propionate Treatment Promotes Wound Healing in Carrot Taproots by Enhancing Phenylpropanoid Metabolism and Reactive Oxygen Species Levels [J]. FOOD SCIENCE, 2026, 47(7): 314-321.
[6]	ZHANG Junlei, WANG Teng, LIANG Qing, ZHANG Wenbin, WANG Ran, HUANG Aixiang, WEI Guangqiang. Structural Properties and Transport Mechanism of the Signaling Molecule AI-2 Transporter GXS4 of Limosilactobacillus fermentum [J]. FOOD SCIENCE, 2026, 47(6): 89-98.
[7]	ZHAO Yuhua, JIAO Xinyao, YANG Xue, FENG Huiyong, LI Tianming. Construction of Bacillus subtilis Strain against Contaminating Bacteria Based on Phosphite Dehydrogenase Gene as Selection Marker [J]. FOOD SCIENCE, 2026, 47(6): 145-154.
[8]	GUO Yuanyuan, LI Ting, ZHI Jianying, PENG Zihua, ZHONG Qingping. Anti-biofilm Effects of Lactiplantibacillus plantarum Z139 Extract on Single and Mixed Cultures of Two Foodborne Pathogens [J]. FOOD SCIENCE, 2026, 47(6): 167-174.
[9]	JI Hongfei, HAN Xiao, YANG Qiuning, LU Yihan, JIANG Yanjun, DONG Xiaodan, DING Wenjie, JI Haiyu. Immunomodulatory Activity of Xylan Palmitate from Suillus luteus through Lactobacillus Enrichment in the Gut [J]. FOOD SCIENCE, 2026, 47(4): 139-150.
[10]	TONG Wenhua, WANG Hui, ZHANG Yadong, CHENG Wei, CHEN Bo, LI Yiyun, YANG Ying, HUANG Dan, SHEN Yi, LUO Huibo. Multi-omics Analysis of the Mechanisms of Tetramethylpyrazine Synthesis in Bacillus siamensis and Its Impact on Baijiu Flavor [J]. FOOD SCIENCE, 2026, 47(2): 113-123.
[11]	SUO Linglan, LI Yue, JIANG Bin, LENG Yue, REN Dayong. Effect of Bacillus paralicheniformis Q13 on Alleviating Hyperuricemia Induced by Potassium Oxonate in Mice [J]. FOOD SCIENCE, 2025, 46(9): 177-188.
[12]	HAN Dong, ZHANG Jiahui, JIANG Zhengqiang, CHANG Chang. Structural Characterization and Anti-hyperuricemic Activity of Exopolysaccharides Secreted by Lactiplantibacillus plantarum-30 [J]. FOOD SCIENCE, 2025, 46(9): 148-155.
[13]	ZHAO Kui, GU Xinyue, WEI You, DU Zihao, LI Jianlong, HU Kaidi, LI Qin, ZHAO Ning, LIU Shuliang. Vinegar Quality Deterioration Caused by Gas-Producing Acetilactobacillus jinshanensis and Quality Changes after Heat Treatment [J]. FOOD SCIENCE, 2025, 46(8): 124-130.
[14]	YANG Xiaohua, HONG Jiaqi, WANG Linhao, PAN Liyi, GUO Hezixian, WANG Jie, LIAO Zhenlin. Screening and Evaluation of Lactic Acid Bacteria for Hypoglycemic Effect in Type 2 Diabetic mellitus Mice [J]. FOOD SCIENCE, 2025, 46(8): 151-161.
[15]	LIANG Xinmin, LI Jiaxun, LI Yangfu, QIN Keyuan, LI Xiang, ZHANG Jumei, WANG Juan, WU Qingping, DING Yu. Effect of YjbK, a CYTH-like Protein, on the Biosynthesis of Cereulide in Foodborne Bacillus cereus [J]. FOOD SCIENCE, 2025, 46(7): 125-134.

Preliminary Identification and Analysis of Cellulase-producing Strains from Carrot

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments