FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 233-236.doi: 10.7506/spkx1002-6300-201005052

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Preliminary Identification and Analysis of Cellulase-producing Strains from Carrot

HOU Jin-hui,LIU Quan-de,GAO Ming-xia,GUO Xiang,YE Jun   

  1. College of Food (Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2009-05-27 Online:2010-03-01 Published:2010-12-29
  • Contact: HOU Jin-hui E-mail:houjinhui0@126.com

Abstract:

In this study, a strain, HP1, with high cellulase activity was isolated from the surface of carrots. The 16S rDNA fragment of HP1 was identified to be 1435bp by PCR. The sequence was analyzed by bioinformatics method and aligned with sequences published in NCBI Genbank. This strain exhibited 99% similarity with Bacillus cereus. According to colony observation combined with physiological-biochemical analysis, HP1 was preliminarily identified to belong to Bacillus sp., which was a new strain of Bacillus based on sequence and biochemical analyses. The accession number of this strain in NCBI was FJ908092.

Key words: carrot, cellulase, Bacillus, 16S rDNA

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