Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis

doi:10.7506/spkx1002-6300-201005058

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 256-260.doi: 10.7506/spkx1002-6300-201005058

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Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis

ZHU Yun-lan，CHEN An-hui，WANG Tao，CHEN Hong-wei*

College of Food(Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China

Received:2009-01-04 Revised:2009-09-02 Online:2010-03-01 Published:2010-12-29
Contact: CHEN Hong-wei E-mail:chenhw@xzit.edu.cn

Abstract

Abstract:

Cordyceps sinensis was used as original strain to conduct protoplast mutagenesis with ultraviolet (UV) illumination for obtaining high-performance mutants. Results showed that 30-second UV illumination gave protoplast-regenerated strains of Cordyceps sinensis No.3 with the highest intercellular polysaccharide production and No.8 with the highest biomass and ability to enrich selenium. The intercellular polysaccharide content (calculated on the basis of dry weight of Cordyceps sinensis mycelia) in strain No. 3 reached up to 54.570 mg/g, which was increased by 52.9 % when compared with that in original strain. Strain No. 8 exhibited 1.3445 g/100mL biomass and 125.8 μg/g selenium content (also calculated on the basis of dry weight of Cordyceps sinensis mycelia), which were increased by 55.16% and 50.11%, respectively, when compared with original strain.

Key words: Cordyceps sinensis, protoplast, mutagenesis, strain breeding

CLC Number:

Q935

ZHU Yun-lan，CHEN An-hui，WANG Tao，CHEN Hong-wei*. Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis[J]. FOOD SCIENCE, 2010, 31(5): 256-260.

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Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis

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