Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma

doi:10.7506/spkx1002-6300-201008001

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 1-5.doi: 10.7506/spkx1002-6300-201008001

• Processing Technology • Next Articles

Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma

HUANG Ping-ping1，YANG Ri-fu2，HUANG Xing-xin1，FAN Xiao-dan1，QIU Tai-qiu1,*

1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China；
2. School of Physics, South China University of Technology, Guangzhou 510640, China

Received:2009-06-12 Online:2010-04-15 Published:2010-12-29
Contact: QIU Tai-qiu1 E-mail:tqqiu@scut.edu.cn

Abstract

Abstract:

To prepare high-purity shikonin from Arnebia euchroma grown in Xinjiang autonomous region, subcritical water extraction and macroporous resin adsorption for purification were conducted. Effects of two extraction modes, namely dynamic circulation and static equilibrium, on shikonin yield were examined. Shikonin adsorption/desorption experiments were carried out using different polar macroporous resins and organic solvents. HPLC-ELSD was used to analyze the purity of the product obtained. Results showed that a maximum shikonin yield of 0.216% was achieved through 8 min extraction in the mode of dynamic circulation under the following conditions: extraction pressure 10 MPa, extraction temperature 120 ℃ and flow rate 1.39 cm3/s; in contrast, the 16 min static equilibrium extraction gave a maximum shikonin yield of 0.198%. Among 5 macroporous resins tested, DA-201 resin exhibited the best adsorption performance and an adsorption rate of 89.7% was obtained after 24 h constant temperature shaking adsorption. Among 4 organic solvents tested, acetone was the best one for shikonin desorption and desorbed 93.2% of shikonin at a fast velocity. The purity of shikonin was increased from 27.31% to 85.07% after optimized purification using DA-201 resin and acetone. The above results demonstrate that subcritical water extraction followed by macroporous resin adsorption provides an effective approach to preparing high-purity shikonin from Arnebia euchroma.

Key words: sub-critical water, dynamic circulation, static equilibrium, shikonin, macroporous resin

CLC Number:

TQ645.96

HUANG Ping-ping1，YANG Ri-fu2，HUANG Xing-xin1，FAN Xiao-dan1，QIU Tai-qiu1,*. Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma[J]. FOOD SCIENCE, 2010, 31(8): 1-5.

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Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma

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