FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (2 ): 130-133.doi: 10.7506/spkx1002-6630-200902026

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Study on Extraction and Purification of Polysaccharides from Pholidota chinensis Lindl

YANG Hai-hua1，WENG Han-mei2，GUAN Yong-guang1，LIN Yan2，GAN Chun-ji2,*   

  1. (1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China ；
    2. Biotechnology Centre, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Received:2007-11-27 Revised:2008-09-17 Online:2009-01-15 Published:2010-12-29
  • Contact: GAN Chun-ji2,* E-mail:ganchunji1949@yahoo.com.cn

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Abstract:

Using the methods of single factor test and orthogonal design test of L9(34), the effects of various factors such as the ratio of sample to water, the extraction time and temperature on the extraction of polysaccharides in Pholidota chinensis Lindl were investigated and the optimal conditions were concluded. The polysaccharides were purified by ethanol precipitation and protein salting-out. The results showed that the main factors from the most important to the less affecting the extraction efficiency of polysaccharides are as following: extraction time, ratio of sample to water and extraction temperature. The optimal extracting conditions are established as following: extraction time 5 hours, ratio of sample to water 1:40 and extraction temperature 80 ℃. Under the optimized parameters, the extraction efficiency of polysaccharides in Pholidota chinensis Lindl is 2.25%, and the obtained polysaccharides are identified as pure polysaccharides.

Key words: polysaccharides, Pholidota chinensis Lindl, extraction, purification

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