FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 207-209.doi: 10.7506/spkx1002-6630-200904044

Previous Articles     Next Articles

Construction and Application of Piezoelectric Biosensor for Rapid Detection of Escherichia coli in Milk

LIU Su-qin,DAI Gao-peng,WANG Qi-hui   

  1. Department of Chemistry and Biological Science, Xiangfan University, Xiangfan 441053, China
  • Received:2008-02-29 Revised:2008-09-26 Online:2009-02-15 Published:2010-12-29
  • Contact: LIU Su-qin E-mail:liusuqin888@hotmail.com

Abstract:

An automatic detection instrument based on tandem type piezoelectric biosensor was established, and then a model milk sample inoculated with Escherichia coli was tested by this instrument. Based on the linear relationship between frequency detection time (FDT) and bacterial concentration of milk sample, accurate numbers of counting bacteria were attainable. The results showed that target bacteria may be detected at concentrations ranged from 10 cell/ml to 106 cell/ml, and detection time is 3 to 15 h. The accuracy of this method is similar to the standard plate counting method (r≥0.99), but it is more sensitive, rapider and more convenient.

Key words: piezoelectric biosensor, Escherichia coli, milk

CLC Number: