Study on Antimicrobial Activity and Mechanism of Peptide from Rana chensinensis Skin

doi:10.7506/spkx1002-6630-200905013

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 64-67.doi: 10.7506/spkx1002-6630-200905013

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Study on Antimicrobial Activity and Mechanism of Peptide from Rana chensinensis Skin

LIU Hong-yu1，CUI Hong-bin2，XU Yan1

(1.College of Food Science, Northeast Agricultural University, Harbin 150030, China；
2.College of Public Health, Harbin Medical University, Harbin 150086, China)

Received:2008-03-27 Revised:2008-09-25 Online:2009-03-01 Published:2010-12-29
Contact: LIU Hong-yu1， E-mail:liuhongyu70@yeah.net

Abstract

Abstract:

The peptide from Rana chensinensis skin has broad antibacterial spectrum, and may inhibit Gram-negative, Grampositive bacteria and yeast. The antibacterial activity also shows fairly strong thermal and acid stabilities. But when pH value is above 8, the antibacterial activity disappears. Compared with the intact cell wall of Bacillus subtilis in the control group, the broken and perforated phenomena of cell wall of Bacillus subtilis treated with the peptide was detected with scanning electron microscopy (SEM), which suggested that the antibacterial mechanism of the peptide from Rana chensinensis skin is to damage bacterial wall and result in cytoplasm outflow and bacteria death.

Key words: Rana chensinensis, frog skin peptide, antimicrobial activity

CLC Number:

TS254.4

LIU Hong-yu1，CUI Hong-bin2，XU Yan1. Study on Antimicrobial Activity and Mechanism of Peptide from Rana chensinensis Skin[J]. FOOD SCIENCE, 2009, 30(5): 64-67.

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Study on Antimicrobial Activity and Mechanism of Peptide from Rana chensinensis Skin

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