Abstract:

Objective: To investigated antioxidant activities of Stropharia rugoso-annulata polysaccharides (SRP) in vivo and in vitro, respectively. Methods: The antioxidant activities of SRP in vitro were evaluated by determining its ability to scavenge free radicals and inhibit erythrocyte hemolysis, MDA generation and mitochondria swelling. In animal test, the oxidant damage model was produced by consecutively injecting D-galactose into the hypoderm of mice for 60 days. At the same time the mice were administered orally with three dosages of SRP (100, 200 and 400 mg/kg·b, respectively), and the levels of SOD and MDA in blood were regularly measured. After mice were sacrificed, their blood and liver samples were collected for assaying the antioxidant indexes. Results: in vitro SRP shows excellent antioxidant activities. The IC50 values of SRP to scavenge superoxide radicals is 132.5 μg/ml, the scavenging rate of 583.1 μg/ml SRP to hydroxyl radicals is 47.44%, the inhibitory rate of 832 μg/ml SRP to erythrocyte homolysis induced by H2O2 is 23.8%, the inhibitory rate of 500 μg/ml SRP to mitochondria swelling induced by Fe2++VC is as good as the control group, and the inhibitory rates of 537 μg/ml SRP to liver MDA generation under autoxidation and induced oxidation conditions are 38% and 40.8% respectively. In the animal test, SRP treatment has no effect on the body weight of model mice, but makes the decreased hemoglobin (Hb) content and liver index in model mice recover to the normal range. Compare with the model group, SRP (200 mg/kg) treatment significantly promotes blood SOD activity on the 20th, 40th, 60th day and reduces blood MDA content on the 40th and 60th day after administering. Moreover, SRP treatment significantly reduces serum and liver MDA contents and promotes activities of serum GPx and TAOC and liver endogenous antioxidant enzymes (total SOD, Cu,Zn-SOD, Mn-SOD, GPx and TAOC) of model mice. Conclusion: SRP possesses excellent antioxidant activities of scavenging free radicals in vitro and D-galactose induced oxidative damage in vivo.

Key words: Stropharia rugoso-annulata polysaccharides (SRP), free radical, oxidative damage model, antioxidation