Study on Enzymatic Synthesis of Ethyl Caproate in Organic Medium

doi:10.7506/spkx1002-6630-200906025

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 123-127.doi: 10.7506/spkx1002-6630-200906025

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Study on Enzymatic Synthesis of Ethyl Caproate in Organic Medium

ZENG Jia-yu1,2，ZHOU Xing-hui1，ZHANG Ji1,2，TANG Gong1，GAO Ya-juan1

(1. College of Life Science, Northwest Normal University, Lanzhou 730070, China；
2. Key Laboratory of Polymer Materials of Gansu Province, Lanzhou 730070, China)

Received:2008-06-02 Revised:2008-08-28 Online:2009-03-15 Published:2010-12-29
Contact: ZENG Jia-yu1,2 E-mail:zengjy@nwnu.edu.cn

Abstract

Abstract:

The synthesis of ethyl caproate catalyzed by candida rugosa lipase (CRL) in organic solvents was investigated, and the effects of reaction time, organic solvent, substrate concentration, and the molar ratio of acid to alcohol on ethyl caproate synthesis were examined systematically. Moreover, the structure, purity and molecular weight of the synthesized product were also characterized by IR, GC and GC-MS. The experimental results showed that the synthesized product is ethyl caproate, its molecular weight is 144, and its purity reaches 96.91%. When the esterification is carried out with the molar ratio of acid to ethanol of 1:1.3 at 38 ℃ for 24 h by using n-heptane as reaction medium, the yield of the esterification reaches 94.6%.

Key words: lipase, ethyl caproate, organic medium, esterification

CLC Number:

TS264.3

ZENG Jia-yu1,2，ZHOU Xing-hui1，ZHANG Ji1,2，TANG Gong1，GAO Ya-juan1. Study on Enzymatic Synthesis of Ethyl Caproate in Organic Medium[J]. FOOD SCIENCE, 2009, 30(6): 123-127.

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Study on Enzymatic Synthesis of Ethyl Caproate in Organic Medium

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