Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method

doi:10.7506/spkx1002-6630-200906056

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 238-242.doi: 10.7506/spkx1002-6630-200906056

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Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method

BAI Wei-bin1,2，SUN Jian-xia1,2，TRINH Quoc-linh1，LUO Yun-bo1，JIANG Gui-chuan2，HUANG Ya-dong3,*

(1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
2. Shandong Agricultural Administrators College, Jinan 250100, China；
3. Biopharmaceutic R and D Center of Jinan University, Guangzhou 510630, China)

Received:2008-05-09 Revised:2008-08-28 Online:2009-03-15 Published:2010-12-29
Contact: BAI Wei-bin1,2 E-mail:baiweibin@163.com

Abstract

Abstract:

A real-time fluorescence PCR method was developed for quantitative detection glyphosate-resistant genetically modified soybean powder. The detection results showed that the genetically modified (GM) contents of soybean powder from USA, Argentina, Brazil and China are 5.37%, 3.96%, 2.26% and 0, respectively. The sensitivity of established detection method is 0.001% and its stability is fairly good.

Key words: genetically modified soybean powder, real-time fluorescence PCR, detection, sensitivity, stability

CLC Number:

TS207.7

BAI Wei-bin1,2，SUN Jian-xia1,2，TRINH Quoc-linh1，LUO Yun-bo1，JIANG Gui-chuan2，HUANG Ya-dong3,*. Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method[J]. FOOD SCIENCE, 2009, 30(6): 238-242.

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Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method

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