Preliminarily Study on Rapid Culture of Escherichia coli O157:H7

doi:10.7506/spkx1002-6630-200907033

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 145-147.doi: 10.7506/spkx1002-6630-200907033

Previous Articles Next Articles

Preliminarily Study on Rapid Culture of Escherichia coli O157:H7

LAI Wei-hua1, 2 Daniel Y C Fung2,* Beth Ann Crozier-Dodson2

(1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2.Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA)

Received:2008-07-12 Revised:2008-11-28 Online:2009-04-01 Published:2010-12-29
Contact: Daniel Y C Fung2,* E-mail:dfung@ksu.edu

Abstract

Abstract:

In this study, oxyrase was added into broth that was inoculated with Escherichia coli O157:H7 to accelerate the growth of this facultative anaerobic microorganism. It was found that the Escherichia coli O157:H7 concentration in the broth added with oxyrase is much more than that of control (no oxyrase added), indicating that oxyrase has certain potential application value for rapid detection of Escherichia coli O157:H7.

Key words: oxyrase, facultative anaerobic microorganism, Escherichia coli O157:H7

CLC Number:

TS207.3

LAI Wei-hua1, 2 Daniel Y C Fung2,* Beth Ann Crozier-Dodson2. Preliminarily Study on Rapid Culture of Escherichia coli O157:H7[J]. FOOD SCIENCE, 2009, 30(7): 145-147.

[1]	SHAN Shan, HUANG Yanmei, ZHAO Xuelong, CHEN Wenyao, LIU Chengwei, LONG Zhong’er, LIU Daofeng,. Detection of Escherichia coli O157:H7 by Cascade Signal Transduction System Combined with Immunochromatography [J]. FOOD SCIENCE, 2021, 42(18): 314-320.
[2]	WU Lina, DONG Pengcheng, ZHANG Yimin, MAO Yanwei, LIANG Rongrong, YANG Xiaoyin, ZHU Lixian, LUO Xin. Biofilm Formation Ability of Escherichia coli O157:H7 on Stainless Steel Surface and Its Relationship with Bacterial Characteristics [J]. FOOD SCIENCE, 2020, 41(22): 127-132.
[3]	YU Lanlin, JI Saisai, YU Jinlong, FU Wenjing, ZHANG Lin, LI Jiaolong, GAO Feng, JIANG Yun. Effects of Salt Stress on the Survival and Virulence Genes Expression of Escherichia coli O157:H7 [J]. FOOD SCIENCE, 2020, 41(14): 95-101.
[4]	WANG Xianjing, DONG Chen, YU Jinlong, HU Jie, FU Wenjing, ZHANG Su, JIANG Yun. Effect of Acid Stress on Biofilm Formation of Escherichia coli O157:H7 [J]. FOOD SCIENCE, 2019, 40(19): 1-7.
[5]	WANG Shujuan, LIU Cheng, FANG Shuiqin, TIAN Yachen, DONG Qingli, WANG Xiang, XU Dongpo, LIU Qing,. Rapid Quantitative Detection of Escherichia coli O157:H7 in Milk Based on Catalytic Activity of Cobalt-Based Metal Organic Framework (ZIF-67) [J]. FOOD SCIENCE, 2019, 40(18): 323-328.
[6]	LIU Siyuan, GU Shaopeng, SUI Zhiwei, LIU Sizhang, XUE Lei, HUO Nairui. Rapid and Quantitative Detection of Escherichia coli O157:H7 in Milk by Flow Cytometry [J]. FOOD SCIENCE, 2018, 39(6): 302-306.
[7]	JI Saisai, WANG Xianjing, MA Jingjing, JIANG Xiaoyan, YUAN Huanyun, YU Jinlong, JIANG Yun. Survival of Frozen Escherichia coli O157:H7 after Thawing [J]. FOOD SCIENCE, 2018, 39(6): 82-87.
[8]	KONG Xiaoxue, HAN Yanqing, FU Yong, JI Saisai, WANG Xianjing, YU Jinlong, JIANG Yun. Flow Cytometric Assessment of Sublethal Injury of Escherichia coli O157:H7 Cells Caused by High Hydrostatic Pressure [J]. FOOD SCIENCE, 2018, 39(3): 135-141.
[9]	KONG Xiaoxue, FU Yong, JI Saisai, JIANG Yun, ZHANG Zhiguo. Cell Membrane Injury of Escherichia coli O157:H7 Caused by Hydrostatic Pressure [J]. FOOD SCIENCE, 2017, 38(8): 1-5.
[10]	SHUI Xinyun1, WANG Huhu2, GAO Feng2, JIANG Yun1,*. Detection of Virulence Genes of Four Escherichia coli O157:H7 Strains and Cold Stress Injury in the Bacteria [J]. FOOD SCIENCE, 2016, 37(4): 176-180.
[11]	XU Yi-gang1, LI Dan-dan2, CUI Li-chun3, LIU Zhong-mei1, LI Su-long1. Detection of Enterohemorrhagic Escherichia coli O157:H7 by DPO-PCR Method [J]. FOOD SCIENCE, 2014, 35(8): 160-164.
[12]	LI Hao-lin1,2, LIU Qing2, LIU Fang3, DONG Qing-li2, QIU Shi2, YANG Yu-ping2, GUO Hui-qing4, HAN Shun-yu1,*. Quantitative Detection of Escherichia coli O157:H7 Based on Visual Antibody Macroarray Technology [J]. FOOD SCIENCE, 2014, 35(20): 185-191.
[13]	SHAN Shan, LAI Wei-hua*, CHEN Ming-hui, CUI Xi. Research Progress in Sources and Distribution of Escherichia coli O157:H7 in Agricultural Products [J]. FOOD SCIENCE, 2014, 35(1): 289-293.
[14]	XIE Quan-yuan，LAI Wei-hua，LIU Chun-mei，SUN Ji-chang，DENG Sheng-liang，LIU Cheng-wei，WEI Hua，YOU Xing-yong，XIONG Yong-hua. Development of Fluorescent Microsphere Immunochromatographic Strip for Detection of Escherichia coli O157:H7 [J]. FOOD SCIENCE, 2013, 34(16): 353-357.
[15]	WANG Li-jun，XU Heng-yi*，XIONG Yong-hua，LI Ping. Application of Real-time Fluorescent Quantitative Polymerase Chain Reaction in Detection of Escherichia coli O157:H7 [J]. FOOD SCIENCE, 2013, 34(11): 358-362.

Preliminarily Study on Rapid Culture of Escherichia coli O157:H7

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments