FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 177-180.doi: 10.7506/spkx1002-6630-200907040

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Study on Mutagenesis of Nisin-producing Strain Induced by Low Energy N+ Implantation

CHEN Hong-wei CHEN Lin-hai* ZHANG Guo-zhi   

  1. (Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China)
  • Received:2008-06-06 Online:2009-04-01 Published:2010-12-29
  • Contact: CHEN Lin-hai* E-mail:chenlinhai@zzu.edu.cn

Abstract:

In this study, the nisin-producing strain Lactococcus lactis J7 was mutagenized using low energy N+ implantation and UV radiation, and its mutant strain Lactococcus lactis K25 was obtained. The nisin potency is increased from 1732.45 IU/ml to 2291.75 IU/ml, by 32.3% through the mutagenesis of Lactococcus lactis J7. Moreover, Lactococcus lactis K25 shows nice genetic stability.

Key words: nisin, ion beam implantation, mutagenesis

CLC Number: