FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 181-185.doi: 10.7506/spkx1002-6630-200907041

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Optimization of Production Conditions of Phenylalanine Ammonia-Lyase by Recombinant E.coli

CUI Jian-dong ZHANG Yun- feng LI Yan   

  1. (Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and
    Technology, Shijiazhuang 050018, China)
  • Received:2008-06-09 Revised:2008-10-30 Online:2009-04-01 Published:2010-12-29
  • Contact: CUI Jian-dong E-mail:cjd007cn@163.com

Abstract:

Effects of initial pH value of fermentation medium, inoculation time, inoculation volume, broth volume in 250-ml flask and fermentation time on the activity of phenylalanine ammonia-lyase (PAL) produced by recombinant E. coli JM109 were investigated through single-factor test, and then the main affecting factors confirmed in single-factor test were optimized through response surface analysis (RSA). The results showed that initial pH value of fermentation medium and broth volume in 250-ml flask are not main affecting factors, and they can be controlled at 6.5 to 7 and 50 ml, respectively; inoculation time, inoculation volume and fermentation time are main affecting factors, and their optimal levels are 13.3 h, 10.7 % and 25.5 h, respectively. By this optimization, the activity of PAL is increased by about 15%, reaching 14.95 U/ml.

Key words: phenylalanine ammonia-lyase, recombinant E. coli, culture conditions, response surface analysis

CLC Number: