FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 120-123.doi: 10.7506/spkx1002-6630-200908023

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Study on Extraction Technology of Algae Oil from Chlorella

LIU Sheng-chen,ZOU Ning*,SUN Jie,SUN Dong-hong,LIANG Yan   

  1. (College of Life Sciences, Ludong University, Yantai 264025, China)
  • Received:2008-07-01 Online:2009-04-15 Published:2010-12-29
  • Contact: ZOU Ning*, E-mail:ningzou76@hotmail.com

Abstract:

The repeated freezing and thawing technique was taken to break cell wall of chlorella and then the algae oil was extracted with ethanol. The effects of ethanol concentration, ratio of material to solvent, times of freezing and thawing, and extraction temperature on the oil yield were investigated. The results showed that the optimum ethanol concentration, ratio of solvent to material, times of freezing and thawing, and extraction temperature are 95%, 3:1 (V/W), once, and 45 ℃, respectively. Under these conditions, the algae oil yield reaches 24.28%. Compared to traditional methods, the combined method of repeated freezing-thawing and ethanol extraction can completely extract the algae oil with a high yield, and is suitable for large-scale industrial production.

Key words: chlorella, algae oil, extraction, repeated freezing and thawing, cell fragmentation

CLC Number: