Abstract:

Objectives: To establish a GC-MS method for determing of essential oil from Grateloupia filicina and to confirm the effect of drying on the chemical constituents in the essential oil. Methods: The essential oil were extracted from fresh and dried Grateloupia filicina at 40℃ in essential oil analyzer with ethyl acetate, and then determined using electron impact mass spectrometry under full scanning mode (EI-SCAN-MS) after concentration. The similarity retrieval of the separated constituents was conduct based on two mass spectral databases (NIST98-147 and Wiley7 Mass Spectral Libraries). Results: Fourty seven volatile compounds are contained in fresh and dried Grateloupia filicina, and their relative contents are 43.81 and 22.23 mg/g, respectively. The main constituents in essential oil from Grateloupia filicina are aldehydes, fatty acids and alcohols, and aldehyde kind is the most. For both two essential oils, the contents of palmitic acid and pentadecanain are the highest in the fatty acids and the aldehydes, respectively. Drying has no any effects on the identified volatile compound kind contained in Grateloupia filicina, and the constituents with short retention time exhibit increasing trend after drying of Grateloupia filicina, while those with long retention time decrease.

Key words: Grateloupia filicina, drying, essential oil, GC-MS