Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC

doi:10.7506/spkx1002-6630-200910045

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 203-205.doi: 10.7506/spkx1002-6630-200910045

Previous Articles Next Articles

Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC

TIAN Hong-zhe，ZHOU Yan-ming，ZHAO Ying-bo，ZANG Jian，HU Rui

(Analyzes and Testing Center, Shenyang Agricultural University, Shenyang 110161, China)

Received:2008-07-18 Revised:2008-10-13 Online:2009-05-15 Published:2010-12-29
Contact: TIAN Hong-zhe， E-mail:tthz2@yahoo.com.cn

Abstract

Abstract:

A method for determining rutin in Rosa roxburghii Tratt fruit by high performance liquid chromatography (HPLC) was developed. The powder of Rosa roxburghii Tratt fruit was extracted with ethanol under the assistance of ultrasonic, and the rutin extract was filtrated, concentrated by a rotary evaporator and centrifuged. The supernatant was filtered through a 0.45 μm filter membrane, diluted with mobile phase, and analyzed by reversed-phase liquid chromatography. The linear range of this method was 0.1 to 200 μg/ml, and the determination coefficient was 0.9988. The precision RSD was 2.10% (n = 5). The average spike recovery of rutin was 94.21%, and the RSD was 2.34%.

Key words: Rosa roxburghii Tratt, rutin, quantitative analysis

CLC Number:

R284.1

TIAN Hong-zhe，ZHOU Yan-ming，ZHAO Ying-bo，ZANG Jian，HU Rui. Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC[J]. FOOD SCIENCE, 2009, 30(10): 203-205 https://doi.org/10.7506/spkx1002-6630-200910045

[1]	ZHAO Shiru, HUANG Pujie, LI Xianxiu, MAO Yangchen, WANG Zhenzhen, MAO Jianwei, SHA Ruyi, SHAO Yihuai. Mid-infrared Spectroscopy for Rapid Determination of Key Component Contents in Anji Golden Flower Tea during Eurotium cristatum Fermentation [J]. FOOD SCIENCE, 2026, 47(1): 177-184.
[2]	NONG Jinmei, HAN Mo, WU Siqing, LI Libing, DENG Houqin. Determination of Relative Contents of Isoflavones in Radix Puerariae Extract by Mass Spectrometry Guided High Performance Liquid Chromatography Using Quantitative Analysis of Multi-component with Single Marker [J]. FOOD SCIENCE, 2025, 46(8): 259-266.
[3]	ZHOU Ting, WANG Haige, ZHANG Jixing, BAO Yingcai, YU Yongli, CHANG Jie. Research Progress in Natural Sources, Biosynthesis and Metabolism Regulation of Rutin in Plant-Derived Food Materials [J]. FOOD SCIENCE, 2025, 46(3): 284-295.
[4]	WANG Zhu, WANG Nana, QUAN Wenxuan, YANG Zhannan, LI Chaochan, ZHAO Chao. Recent Advances in Bioactive Compounds, Pharmacological Actions, and Product Development of Major Edible Rosa Fruits [J]. FOOD SCIENCE, 2025, 46(21): 53-62.
[5]	WEI Ye, CAO Jiawei, WANG Aili, ZOU Liang, HUA Xubin, LIN Qiao, QIU Ju. Effects of Different Thermal Treatments on the Rutin Form Distribution and Sensory Quality of Tartary Buckwheat [J]. FOOD SCIENCE, 2025, 46(11): 272-280.
[6]	WANG Qiwen, LUO Qing, XU Yangyang, WANG Ximin, ZENG Congyan, DAI Weibo, PENG Weiwen, DONG Gengting. Protective Effect and Mechanism of Rosa roxburghii Tratt Polysaccharides on Isoprenaline-Induced Myocardial Ischemia in Mice [J]. FOOD SCIENCE, 2024, 45(5): 78-85.
[7]	LIU Haipeng, LI Ling, HAN Jiali, JIANG Xin, FENG Lei, WANG Jia. Preparation and Characterization of Modified Guar Gum/Whey Protein/Rutin Composite Membrane and Its Effect on Storage Quality of Agaricus bisporus [J]. FOOD SCIENCE, 2024, 45(5): 233-242.
[8]	ZHANG Pan, WANG Lei, CHEN Jie, XU Fei. Effects of Polysaccharides from Rosa roxburghii Tratt Fruit on Ileal Mucosal Barrier Function in Mice with Non-alcoholic Fatty Liver [J]. FOOD SCIENCE, 2023, 44(3): 127-136.
[9]	YUE Wei, MENG Lixiu, WANG Yuan, QIN Yongwei. Detection of Rutin in Flower Buds of Sophora japonica by Using Chitosan-Based Carbon Dot Paper Chip [J]. FOOD SCIENCE, 2023, 44(16): 260-266.
[10]	ZUO Yunyang, HU Ping, XU Haoxiang, FENG Dandan, SHI Yuanyuan, LI Jiuchang, WANG Ling, WEI Maoyang. Relieving Effect of Rosa roxburghii Tratt. Juice Fermented by Lactobacillus paracasei SR10-1 on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice [J]. FOOD SCIENCE, 2023, 44(15): 121-128.
[11]	FAN Lixin, MA Junmei, HE Liangna, REN Xiaowei, JIA Wenxuan, LI Qiang. Determination of 11 Foodborne Stimulants and Five Alkaloids in Hot Pot Soup Base Seasoning Using Ultra-high Performance Liquid-Quadrupole-Time of Flight Mass Spectrometry [J]. FOOD SCIENCE, 2023, 44(12): 360-367.
[12]	DING Desheng, WANG Longqiong, HE Xiaoling, PANG Wenxi, LI Yunchang, WANG Yuankai, ZHOU Fenglan, CAI Tian, CHEN Kewei. Analysis of Distribution of Vitamin D and Its Esters in Human Milk Using Ultra-high Performance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry [J]. FOOD SCIENCE, 2023, 44(10): 324-331.
[13]	GUAN Yinlong, ZHOU Liyan, WANG Hui, CHEN Jiayu, MING Jian, LI Fuhua. Impact of Dynamic High Pressure Microfluidization on Dietary Fiber from Rosa roxburghii Tratt.Pomace and Its Inhibitory Capacity against Starch Digestion and Glucose Diffusion [J]. FOOD SCIENCE, 2022, 43(9): 79-86.
[14]	XUE Ailian, LI Chunyi, WANG Qiming, ZHANG Chi, LEI Xiaojuan, ZHAO Jichun, ZENG Kaifang, MING Jian. Effect of Ultrasonic Treatment on the Interaction and Structural Properties of Gliadin-Rutin Complex [J]. FOOD SCIENCE, 2022, 43(7): 45-51.
[15]	KANG Chaodi, WANG Shouwei, ZHANG Yingying, ZHANG Mingyue, ZHAO Wentao, GU Jin, LI Yingying. Quantitative Analysis of Adulterants in Beef by Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2022, 43(4): 270-276.

Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments