FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 203-205.doi: 10.7506/spkx1002-6630-200910045

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Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC

TIAN Hong-zhe,ZHOU Yan-ming,ZHAO Ying-bo,ZANG Jian,HU Rui   

  1. (Analyzes and Testing Center, Shenyang Agricultural University, Shenyang 110161, China)
  • Received:2008-07-18 Revised:2008-10-13 Online:2009-05-15 Published:2010-12-29
  • Contact: TIAN Hong-zhe, E-mail:tthz2@yahoo.com.cn

Abstract:

A method for determining rutin in Rosa roxburghii Tratt fruit by high performance liquid chromatography (HPLC) was developed. The powder of Rosa roxburghii Tratt fruit was extracted with ethanol under the assistance of ultrasonic, and the rutin extract was filtrated, concentrated by a rotary evaporator and centrifuged. The supernatant was filtered through a 0.45 μm filter membrane, diluted with mobile phase, and analyzed by reversed-phase liquid chromatography. The linear range of this method was 0.1 to 200 μg/ml, and the determination coefficient was 0.9988. The precision RSD was 2.10% (n = 5). The average spike recovery of rutin was 94.21%, and the RSD was 2.34%.

Key words: Rosa roxburghii Tratt, rutin, quantitative analysis

CLC Number: