FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 212-214.doi: 10.7506/spkx1002-6630-200910048

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Determination of Six Benzimidazoles Residues in Pork by SPE-HPLC

LIU Yong-jun,WU Yin-liang,JIANG Yan-bin,LIU Xing-guo,WANG Hai   

  1. (Quality Control and Inspection Center for Domestic Animal Products, Ministry of Agriculture, Beijing 100125, China)
  • Received:2008-07-25 Revised:2008-10-13 Online:2009-05-15 Published:2010-12-29
  • Contact: LIU Yong-jun, E-mail:liuyongjun@eyou.com;liuyongjun37@hotmail.com

Abstract:

Pork sample was extracted with ethyl acetate and purified by SPE- HLB cartridge. The analytes were further separated by HPLC and determined using PDA detector and quantified using external standard method. The results showed that there were good linear relationships between the peak areas and the concentrations of six benzimidazoles in the range of 20 to 1000 ng/ml (r> 0.99). The average spike recoveries of six benzimidazoles were 68.5% to 83.7% at the spiked levels of 0.05, 0.1 and 0.15 mg/L for blank sample, and the precision RSDs were 1.6 to 7.3.

Key words: high performance liquid chromatography, oxfendazole, thiabendazole, cambendazole, mebendazole, albendazole, fenbendazole

CLC Number: