Isolation and Identification of High-yield Melanin-producing Actinomycetes G-HD-4

doi:10.7506/spkx1002-6630-200911041

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (11): 185-188.doi: 10.7506/spkx1002-6630-200911041

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Isolation and Identification of High-yield Melanin-producing Actinomycetes G-HD-4

LIU Xin1，MA Cheng-jie1,2，YANG Shuang-xi1，DU Zhao-ping1，MA Ai-min1,*

(1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China；
2.Wuhan Research Institute, Bright Dairy and Food Co. Ltd., Wuhan 430040, China)

Received:2008-10-10 Revised:2009-01-12 Online:2009-06-01 Published:2010-12-29
Contact: MA Ai-min1,* E-mail:aiminma@mail.hzau.edu.cn

Abstract

Abstract:

Actinomyceter strain G-HD-4 was isolated from soil sample collected from Huaqiao flower garden in Quanzhou of Fujian Province, which produced the most melanin compared to other isolated 18 strains. The melanin separated from the fermentation broth of strain G-HD-4 was characterized by ultraviolet-visible (UV-Vis) spectroscopy, infrared (IR) spectroscopy, and scanning electron microscope (SEM). The results revealed that the melanin derived from strain G-HD-4 was similar to standard melanin on the structural basis. Based on the studies of physiological and biochemical characteristics and 16S ribosomal RNA sequence homology, the G-HD-4 strain was confirmed to belong to Streptomyces genus and be similar to Streptomyces capoamus (gene sequence similarity 99%). Finally, it was identified as a species of Streptomyces capoamus by taxonomic and phylogentic analysis.

Key words: Streptomycs G-HD-4, melanin, 16S rDNA sequence, phylogenetic analysis

CLC Number:

TS252.54

LIU Xin1，MA Cheng-jie1,2，YANG Shuang-xi1，DU Zhao-ping1，MA Ai-min1,*. Isolation and Identification of High-yield Melanin-producing Actinomycetes G-HD-4[J]. FOOD SCIENCE, 2009, 30(11): 185-188.

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Isolation and Identification of High-yield Melanin-producing Actinomycetes G-HD-4

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