Abstract:

Objective: To optimize the parameters of supercritical CO2 extraction of C21 seroidal glycosides from Cynanchum bungei Decne. root by orthogonal design. Methods: Five affecting factors such as extraction pressure, extraction temperature, extraction duration, particle size of material and 95% ethanol (entrainer) dosage were investigated by single factor test, and each factor was assigned with four levels for orthogonal design. Absorbance at 590 nm was taken to represent C21 seroidal glycoside content as the index to determine the optimal extraction conditions. Results: The Temperature, the particle size of material and the 95% ethanol dosage were the main factors affecting the extraction of C21 steroidal glycosides, while the extraction duration presented the smallest effect. The optimal extraction conditions were as follows: pressure, 30 MPa; particle size of material, 40 mesh; 95% ethanol dosage, 2.0 ml/g; temperature 55 ℃; and extraction duration 90 min.

Key words: Cynanchum bungei Decne. root, C21 steroidal glycosides, supercritical CO2 extraction, orthogonal test