Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine

doi:10.7506/spkx1002-6630-200912058

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 254-257.doi: 10.7506/spkx1002-6630-200912058

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Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine

ZHANG Guo-hua，LAI Wei-hua，XIONG Yong-hua，CHEN Yuan

(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)

Received:2008-07-21 Online:2009-06-15 Published:2010-12-29
Contact: ZHANG Guo-hua， E-mail:zgh_206@163.com

Abstract

Abstract:

The quantum dot labeling technique was applied to the development of immunochromatographic test strip for rapid detection of ractopamine. Four steps were involved in the procedure of preparation of the strip, including the preparation of antigen and antibody against ractopamine, the production of quantum dots, and the installation and testing of the strip. The detection limit for ractopamine by this strip was 3 ng/ml, and the detection could be finished within 10 min. Apart from being time-saving, the strip is easy to perform and relatively inexpensive. It is therefore ideally suited for on-site testing of ractopamine.

Key words: ractopamine, quantum dots, immunochromatographic test strip

CLC Number:

TS207.7

ZHANG Guo-hua，LAI Wei-hua，XIONG Yong-hua，CHEN Yuan. Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine[J]. FOOD SCIENCE, 2009, 30(12): 254-257.

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Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine

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