Cellulase-catalyzed Hydrolysis of Cellooligosaccharides

doi:10.7506/spkx1002-6630-200913050

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13): 218-222.doi: 10.7506/spkx1002-6630-200913050

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Cellulase-catalyzed Hydrolysis of Cellooligosaccharides

PENG Hong1,2,3，LIN Lu2，LIU Yu-huan1,3，RUAN Rong-sheng1,3

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China；
3. Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China

Received:2008-10-07 Revised:2009-01-11 Online:2009-07-01 Published:2010-12-29
Contact: PENG Hong1,2,3， E-mail:penghong@ncu.edu.cn; penghong.scut@yahoo.com.cn

Abstract

Abstract:

The cellulase from Aspergillus niger was used to hydrolyze cellotriose, cellotetraose and cellopentaose. The hydrolysis characteristics of the three cellooligosaccharides with different degrees of polymerization (DPs) were compared to understand the effect of DP on cellulose-catalyzed of cellooligosaccharides and explain the hydrolysis law. The results showed that the relative degree of hydrolysis (DH) decreased with the increase of DP. The oligosaccharides in intermediate products and in substrate showed competitive reaction with the enzyme and the oligosaccharides with lower DP displayed priority in the hydrolysis. Excessive usage of enzyme was unable to markedly enhance the hydrolysis. The elevated initial substrate concentration led to the decrease of relative DH. The optimum working temperatures was 55 ℃ for Aspergillus niger cellulase at pH 5.0.

Key words: cellooligosaccharides, cellulase, hydrolysis, degree of hydrolysis, degree of polymerization

CLC Number:

Q556.2

PENG Hong1,2,3，LIN Lu2，LIU Yu-huan1,3，RUAN Rong-sheng1,3. Cellulase-catalyzed Hydrolysis of Cellooligosaccharides[J]. FOOD SCIENCE, 2009, 30(13): 218-222.

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Cellulase-catalyzed Hydrolysis of Cellooligosaccharides

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