FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13 ): 253-255.doi: 10.7506/spkx1002-6630-200913058

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Cultivation of Rat Preadipocytes and Effects of Glycomacropeptide on Its Proliferation

XU Shi-ping,MAO Xue-ying*,REN Fa-zheng   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2009-03-16 Online:2009-07-01 Published:2010-12-29
  • Contact: MAO Xue-ying*, E-mail:wumaoxy@163.com

Abstract:

Preadipocytes were isolated from adipose tissue of SD rats by enzyme digestion, and their cellular morphology and growth curve were analyzed. Furthermore, preadipocytes were induced to differentiation and identified by Oil Red O staining. The results showed that this method can obtain preadipocytes with rapid proliferation and high rate of inducible adipogenic differentiation. Besides, this study also focused on the effects of glycomacropeptide (GMP) isolated from whey protein concentrate on the proliferation of preadipocytes. It was found that the GMP can inhibit the proliferation of preadipocytes.

Key words: preadipocytes, primary cultication, identification, glycomacropeptide

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