Abstract:

Total flavonoids were extracted from dried Potentilla chinensis Ser. powder by ultrasonic-assisted extraction method and purified using macroporous adsorption resin. The ultrasonic-assisted extraction technique was optimized using orthogonal array design. Adsorption and desorption performance of 8 resins to total flavonoids from Potentilla chinensis Ser. were compared to select a optimal one and total flavonoids desorption efficiency of different concentration ethanol aqueous solutions was compared, moreover adsorption and desorption characteristics of the total flavonoids on HPD-600 type resin were investigated. The optimal extraction conditions of total flavonoids were as follows: using 60% ethanol as extraction solvent, material/liquid ratio (m/V) 1:40, extraction duration 75 min, and temperature 80 ℃, and these factors all had statistically significant effects (p ＜ 0.05) on yield of total flavonoids from Potentilla chinensis Ser. that reached 39.329 mg/g under such conditions. Compared to other 7 resins, HPD-600 type macroporous resin had better adsorption and desorption performance. The purification conditions of crude total flavonoid extract using this resin (quantity: 50 ml) were as follows: sample quantity 40 BV, sample flux 2 BV/ h, using 60% ethanol as eluent, and eluent quantity 5 BV, and after the purification, the purity of total flavonoids reached 60.28% and the yield of total flavonoid product was 2.29%.

Key words: Potentilla chinensis Ser., flavonoids, ultrasonic-assisted extraction, macroporous resin purification