Abstract:

The physical and chemical properties of glutamine-bioactive peptides (GBP) which were prepared by hydrolyzing wheat protein by bienzyme method, including solubility, osmotic pressure, content of NaCl and relative molecular mass, were investigated and their antioxidation and anti-fatigue effects of glutamine-bioactive peptides on mice were evaluated. SPF Kunming mice were divided into four groups: three experimental groups (low-, medium- and high-dose groups) administered with different doses of GBP by gavage and control group administered with the same volume of normal saline instead of GBP. Gavage administration was performed in two batches: in the first batch, after 20 days of continued administration, the activity of SOD and the content of MDA in liver and kidney were measured; in the second batch, after 21 days of continued administration, the swimming time and the survival time of mice were measured by swimming test and hypoxia tolerance test, respectively. In comparison with the control group，high-dose GBP could obviously increase the activity of SOD in liver and kidney, reduce the content of MDA, prolong the swimming time and the hypoxia survival time of mice, and enhance the anti-fatigue ability.

Key words: glutamine-bioactive peptides, solubility, osmotic pressure, antioxidation, anti-fatigue