FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 327-332.doi: 10.7506/spkx1002-6630-200918076
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HU Hui-ping,PAN Ying-jie,LIU Yuan,SUN Xiao-hong,ZHAO Yong*
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Microbial volatile metabolites (MVOCs) of three Pseudomonas species isolated from pork during pure culture fermentation in tryptic soy broth (TSB) were analyzed for chemical composition using headspace solid-phase micro extraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) and odor fingerprint using electronic nose (E-nose) for laying a theoretical basis for the rapid and non-destructive detection of microorganisms in foods using odor fingerprint technique. E-nose could clearly distinguish the three Pseudomonas species through principal component analysis (PCA) and discriminant function analysis (DFA). HS-SPME/GC-MS analysis indicated that the MVOCs of Pseudomonas aeruginosa consisted of 2-butanone, dibromochloromethane, isovaleric acid, 2-methylbutanoic acid, 2-methyl-5-(methylthio)furan, Pseudomonas aeruginosa 3,5- dimethyloctane, 6-methyl-5-heptene-2-one, 2-ethylhexanol, dodecane, 2,6,10-trimethyldodecane, (1E)-1-ethylidene-1H-indene, and 1,5-dimethylnaphthalene, and Pseudomonas putida 2-pentanone, methylthioacetate, butyl acetate, S-methyl butanethioate, 1-cycloundecene, pentylcyclopropane, 3-propionylpyridine, and 3-phenylfuran. The combination of E-nose and HS-SPME/GCMS provides a rapid and non-destructive detection method of Pseudomonas spp. and other microorganisms in foods.
Key words: electronic nose (E-nose), headspace solid-phase microextraction/gas chromatography-mass spectrometry (HSSPME- GC-MS), Pseudomonas spp., microbial volatile metabolites (MVOCs)
CLC Number:
TS201.3
HU Hui-ping,PAN Ying-jie,LIU Yuan,SUN Xiao-hong,ZHAO Yong*. Application of Odor Fingerprint for the Detection of Pseudomonas spp. Isolated from Pork[J]. FOOD SCIENCE, 2009, 30(18 ): 327-332.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-200918076
https://www.spkx.net.cn/EN/Y2009/V30/I18 /327