FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 359-361.doi: 10.7506/spkx1002-6630-200918084

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16S rDNA-based Identification of Bifidobacterium in Foods

WU Yong-jun1,2,WANG Jia-fu1   

  1. 1. College of Life Sciences, Guizhou University, Guiyang 550025, China;
    2. Guizhou Key Laboratory of Agricultural-Bioengineering, Guiyang 550025, China
  • Received:2007-11-30 Online:2009-09-15 Published:2010-12-29
  • Contact: WU Yong-jun E-mail:wyjbio@163.com

Abstract:

According to the conservative sequence of16S rDNA from Bifidobacterium genus, two pairs of specific primers were developed, named BifS11 and BifS12, for molecular biological identification of strains of the genus Bifidobacterium by PCR. The combination of ultrasonic treatment and proteinase K-catalyzed hydrolysis resulted in higher purity of genomic DNA than other three methods, and high specificity PCR assays were achieved using the two pairs of primers. The PCR array is rapid and reliable, can provide an efficient detection of Bifidobacterium in foods and probiotics.

Key words: Bifidobateriim, PCR, identification, 16S rDNA

CLC Number: