Antioxidant Activity and DNA Damage Prevention of Polymethoxylated Flavone Extracts from Peels of C. sinensis Osbeck

doi:10.7506/spkx1002-6630-200919002

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19): 19-22.doi: 10.7506/spkx1002-6630-200919002

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Antioxidant Activity and DNA Damage Prevention of Polymethoxylated Flavone Extracts from Peels of C. sinensis Osbeck

YAO Xiao-lin，XU Xiao-yun，DUAN Chun-hong，FAN Gang，PAN Si-yi*

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2009-05-18 Online:2009-10-01 Published:2010-12-29
Contact: PAN Si-yi* E-mail:pansiyi@mail.hzau.edu.cn

Abstract

Abstract:

The antioxidant activity of polymethoxylated flavones (PMF) extracts from peels of C. sinensis was evaluated by chemiluminescence (CL) method in vitro, the scavenging capabilities of PMFs against superoxide anion, hydroxyl free radial, and hydrogen peroxide were determined by Pyrogallol-Luminol system, CuSO4-Phen-VC-H2O2 system, and Luminol-H2O2 system, respectively, and the prevention effect of PMFs on DNA damage was evaluated by CuSO4-Phen-VC-H2O2-DNA CL system. Results showed that PMFs exhibited effective antioxidant activity to scavenge superoxide anion, hydroxyl free radial, hydrogen peroxide, and prevention on DNA damage in a dose-dependent manner. The IC50s of PMFs to superoxide anion, hydroxyl free radial, and hydrogen peroxide were 6.1, 7.8, 0.6 and 4.0 μg/ml, respectively.

Key words: citrus, polymethoxylated flavones (PMFs), antioxidant activity, chemiluminescence

CLC Number:

O623.54

YAO Xiao-lin，XU Xiao-yun，DUAN Chun-hong，FAN Gang，PAN Si-yi*. Antioxidant Activity and DNA Damage Prevention of Polymethoxylated Flavone Extracts from Peels of C. sinensis Osbeck[J]. FOOD SCIENCE, 2009, 30(19): 19-22.

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Antioxidant Activity and DNA Damage Prevention of Polymethoxylated Flavone Extracts from Peels of C. sinensis Osbeck

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