Abstract:

Liposomes were prepared using a microfluidiezer to encapsulate neutral proteases. The shape, distribution in particle size and Zeta potential of liposome-encapsulated neutral proteases, and its physical stability under various pH conditions, processing temperatures and ion concentrations were systematically investigated. Results indicated that the prepared liposomes exhibited spherical shape with even distribution in particle size. The average particle size of liposome was 85.0 nm and Zeta potential was －25.77 mV. A relative stable disperse phase of liposomes was observed in the range of pH from 6.0 to 10.0. However, its stability decreased as the increasing of sodium ion concentration. Heating treatment had no significant effect on the stability of disperse phase of liposomes. The maximum loading capacity for liposome-encapsulated neutral proteases was 16.8%.

Key words: liposome, microfluidizer, neutral protease, stability, Zeta potential