Abstract:

In order to detect genes related to aflatoxin biosynthesis, a high quality gene chip was successfully developed here. The preparation of this gene chip was involved in a series of processes: array on the slide, heating in 42 water bath for 2 h, UV-induced crosslink at 650 mJ/cm2 for 30 seconds, baking in oven at 80 for 2 h, pre-hybridization for 45 min, then washing and drying, and final hybridization with fluor-derivatized samples at 42 for 16 h. The probe originated from Aspergillus parasiticus could be used to analyze aflatoxin gene expression in Aspergillus flavus. Therefore, this probe can provide stable fluorescence signal without background inference and results consistent with reverse transcriptase polymerase chain reaction (RT-PCR).

Key words: Aspergillus parasiticus, Aspergillus flavus, aflatoxin, gene chip