FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 299-302.doi: 10.7506/spkx1002-6630-200924065

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Rapid Test Strip for Coliform Bacteria

ZHANG Li-li1,2，LAI Wei-hua1,*，TU Zu-xin2，QU Feng1   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
    2. Microbiology Institute, Jiangxi Academy of Sciences, Nanchang 330029, China
  • Received:2009-08-15 Online:2009-12-15 Published:2010-12-29
  • Contact: LAI Wei-hua1,*， E-mail:talktolaiwh@163.com

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Abstract:

In this study, rapid test strips with strong specificity and high sensitivity for detecting coliform bacteria were developed through optimizing medium, pH, and addition amount of bromocresol purple in medium. The sensitivity of homemade test strips exhibited a significant increase by adding beef extract at the dose of 3 mg/ml into culture medium, adjusting pH to 6.62 ± 0.2, and adding bromocresol purple at the dose of 0.064 mg/ml. This kind of strip can be used in the food safety inspection to detect coliform bacteria.

Key words: coliform bacteria, rapid test strip, bromocresol purple

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