FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 131-134.doi: 10.7506/spkx1002-6630-201009031

• Bioengineering • Previous Articles     Next Articles

Isolation, Purification and Structural Modification of Exopolysaccharide from Lactobacillus plantarum C21

LI Sheng-yu,ZENG Xian-peng,ZHAO Yu-juan,ZHANG Xue,ZHANG Jian,YANG Zhen-nai*   

  1. Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
  • Received:2009-08-12 Revised:2010-01-18 Online:2010-05-01 Published:2010-12-29

Abstract:

In order to investigate the structure and properties of exopolysaccharide produced by Lactobacillus plantarum C21, a neutral exopolysaccharide was isolated from culture supernatant and purified by ethanol precipitation, DEAE-cellulose ion exchange column chromatography and gel filtration chromatography on Sepharose CL-6B column. The structure of this exopolysaccharide was determined by ion chromatography and FTIR spectroscopy and this exopolysaccharide was designated as PCPc. Results indicated that the PCPc was composed of D-galactose and D-glucose in a molar ratio of 1:2. Sulfated and phosphorylated derivatives of PCPc were also prepared.

Key words: lactic acid bacteria, Lactobacillus plantarum, exopolysaccharide

CLC Number: