FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 206-210.doi: 10.7506/spkx1002-6630-201009046

• Bioengineering • Previous Articles     Next Articles

Optimal Condition for Selenium-rich Yeast Cultivation in Beerwort Media

LIU Jie1,CHEN Zhi2,LI Wei-qi1,XU Lin2,WU Gen-fu1,*   

  1. 1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
    2. Zhejiang Nanxi River Brewery Company Limited, Wenzhou 325100, China
  • Received:2009-10-12 Online:2010-05-01 Published:2010-12-29
  • Contact: WU Gen-fu E-mail:wugenfu@zju.edu.cn

Abstract:

The optimal cultivation condition for yeast growth and selenium accumulation in beerwort media was studied. Results indicated that 2oBx beerwort was a suitable medium for the growth of Saccharomyces cerevisiae and the higher concentration would reduce growth rate by Crabtree effect. The addition of yeast extract in 2oBx beerwort could significantly increase yeast biomass; while the addition of (NH4)2SO4 or KH2PO4 did not. In addition, although glucose addition also could improve yeast biomass, its enhancement effect could be inhibited by simultaneous addition of (NH4)2SO4. Initial selenium concentration in media could remarkably improve yeast biomass and enhance selenium accumulation. At the initial concentration of 10 mg/L Na2SeO3, yeast biomass and accumulated selenium reached up to 2.83 g/L and 266.3 mg/kg, respectively.

Key words: selenium-rich yeast, beerwort, Crabtree effect, Na2SeO3

CLC Number: