FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9 ): 202-205.doi: 10.7506/spkx1002-6630-201009045

• Bioengineering • Previous Articles     Next Articles

Screening and Identification of Cholesterol Oxidase-producing Strains with High Activity

CAI Dan,ZHANG Na-na,LIU Jing-sheng*   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2009-12-30 Online:2010-05-01 Published:2010-12-29
  • Contact: LIU Jing-sheng E-mail:liujs1007@vip.sina.com.cn

Abstract:

Twenty-three strains using cholesterol as the sole carbon source were isolated from nine samples collected from different locations. One strain with high yield of extracellular cholesterol oxidase was screened and named as strain A3. The highest activity of cholesterol oxidase was 525 U/L. The strain A3 was identified as Brevibacterium sp. through analyzing its morphology, physiological and biochemical characteristics.

Key words: cholesterol oxidase, strain, screening

CLC Number: