FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10): 250-253.doi: 10.7506/spkx1002-6630-201010054

• Analysis & Detection • Previous Articles     Next Articles

Simultaneous Extraction and Determination of Chromium (Ⅲ) and Chromium (Ⅵ) in Milk

LI Jing,WANG Yu,CHEN Hua-bao,LIANG Li-na*   

  1. Application Research Center, Dionex China, Beijing 100085, China
  • Received:2009-07-09 Revised:2009-12-11 Online:2010-05-15 Published:2010-12-29
  • Contact: LI Jing


A method was developed for the simultaneous extraction and determination of chromium (Ⅲ) and chromium (Ⅵ) in milk. One pretreatment process was required for chromium (Ⅵ) extraction, the release of chromium (Ⅲ) from organic chromium, chromium (Ⅲ) pre-column derivatization and milk protein sedimentation, which exhibited simplicity, convenience and high extraction efficiency of chromium. The separation of chromium (Ⅲ) and chromium (Ⅵ) was achieved on an IonPac CS5A column within 8 min. Chromium (Ⅲ) and chromium (Ⅵ) were chleated with PDCA and DPC and were detected with a UV-Vis detector at UV and visible wavelengths, respectively. This work provides an accurate and practical method for milk quality evaluation.

Key words: chromium (Ⅲ), chromium (Ⅵ), ion chromatography, UV-Vis detection, milk quality testing

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