FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (11): 51-54.doi: 10.7506/spkx1002-6630-201011011

• Basic Research • Previous Articles     Next Articles

Physico-chemical and Structural Characterization of Rapeseed Polysaccharide WPS-1

ZHU Jian-fei1 CHEN Gang1 TANG Chun-hong1 WU Mou-cheng2   

  1. 1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China
    2. Ocean Food Nutrition Research Institute of Shandong Taixiang Group, Rongcheng 264309, China
  • Received:2009-12-03 Online:2010-06-01 Published:2010-12-29
  • Contact: ZHU Jian-fei E-mail:zhujf@sina.cn

Abstract:

WPS-1 was found to be a non-starch polysaccharide with white and flocculent appearance, in which the contents of neutral sugar, uronic acid and protein were 83.2 %, 6.1% and 3.4%, respectively. Protein in WPS-1 was composed of 13 different amino acids. The major monosaccharide compositions of WPS-1 as determined through FT-IR and GC analyses were Ara (67.7%), Gal (17.8%) and Glc (14.5%). The methylation analysis indicated that the major fragments of WPS-1 were (1→5) and (1→2) linked by Ara residues of α-arabinoses. Four kinds of substituted arabinosyl residues as verified according to the 13C NMR and 1H NMR spectra existed in arabinan fragments of WPS-1.

Key words: rapeseed polysaccharide WPS-1, physico-chemical properties, structure

CLC Number: