Abstract:

In this paper, it reports the results of an evaluation of in vitro antioxidant and antimicrobial activity of respective polysaccharides (PEL and PER) extracted from the fruits of Evodia lenticellata Huang and Evodia rutaecarpa (Juss.) Beth using hot water extraction and subsequent ethanol precipitation. The contents of polysaccharides were determined by phenol-sulphuric acid method. The scavenging capacities of PEL and PER against hydroxyl free radicals (·OH), hydrogen peroxide (H2O2) and 2,2-diphenyl-2-picrylhydrazyl free radicals (DPPH·) were evaluated using mimic chemical systems in vitro. Minimal inhibitory concentration as determined by liquid dilution method on cell culture plates was used for anti-microbial activity evaluation. The results showed that the EC50 values of PEL for scavenging ·OH, H2O2 and DPPH· were 0.8, 0.25  mg/mL and 0.9 mg/mL, respectively; for PER, the EC50 values were 1.0, 0.1 mg/mL and 0.8 mg/mL, respectively. Both PEL and PER exhibited obvious scavenging effects against H2O2 and ·OH. In addition, they were both more competent to scavenge H2O2 than vitamin C at the same concentrations. PER provided a potential antimicrobial agent with stronger bioactivity when compared to PEL under the same conditions.

Key words: Evodia lenticellata Huang, Evodia rutaecarpa (Juss.) Beth., polysaccharide, antioxidant activity, antimicrobial activity