Abstract:

Saponins in the roots of Platycodon grandiflorum were extracted with distilled water under the assistance of ultrasonic, and the extraction process was optimized by using response surface analysis based on Box-Behnken design to examine the effects of extraction conditions including material-to-liquid ratio, length of ultrasonic treatment time and extraction temperature on saponin yield, which were reflected by a quadratic regression model. The results showed that the optimal values of the above extraction conditions were as follows: material-to-liquid ratio 1: 9.75 (g/mL), length of ultrasonic treatment time 31 min and extraction temperature 35 ℃. Under these conditions, the saponin yield was 4.64%, higher than before the optimization.

Key words: Platycodon grandiflorum, saponins, response surface analysis, extraction