Abstract:

The application of AB-8 macroporous resin for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was evaluated in the present study. The static adsorption-desorption process of Folium Ilicis Cornutae total flavonoids on this resin was optimized using single factor and orthogonal array design methods. The results showed that a good separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was achieved using AB-8 macroporous resin. The optimum conditions for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae were found to be: 4 h adsorption at pH 4.5 of the sample solution, followed by 2.25 h desorption using 90% ethanol as desorption solvent added at 25-fold volume of resin weight. Under these optimum conditions, the recovery of Folium Ilicis Cornutae total flavonoids reached up to 90.8% and the purity of the final product obtained was 33.4%.

Key words: Folium Ilicis Cornutae, total flavonoids, AB-8 macroporous resin, separation and purification