Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae

doi:10.7506/spkx1002-6630-201012017

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 76-79.doi: 10.7506/spkx1002-6630-201012017

• Technology Application • Previous Articles Next Articles

Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae

LIU Zhi-xiang，ZENG Chao-zhen，ZHANG Yue

College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China

Received:2009-10-14 Revised:2010-02-04 Online:2010-06-15 Published:2010-12-29
Contact: LIU Zhi-xiang， E-mail:liuzx0927@yahoo.com.cn

Abstract

Abstract:

The application of AB-8 macroporous resin for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was evaluated in the present study. The static adsorption-desorption process of Folium Ilicis Cornutae total flavonoids on this resin was optimized using single factor and orthogonal array design methods. The results showed that a good separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was achieved using AB-8 macroporous resin. The optimum conditions for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae were found to be: 4 h adsorption at pH 4.5 of the sample solution, followed by 2.25 h desorption using 90% ethanol as desorption solvent added at 25-fold volume of resin weight. Under these optimum conditions, the recovery of Folium Ilicis Cornutae total flavonoids reached up to 90.8% and the purity of the final product obtained was 33.4%.

Key words: Folium Ilicis Cornutae, total flavonoids, AB-8 macroporous resin, separation and purification

CLC Number:

R284.2

LIU Zhi-xiang，ZENG Chao-zhen，ZHANG Yue. Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae[J]. FOOD SCIENCE, 2010, 31(12): 76-79 https://doi.org/10.7506/spkx1002-6630-201012017

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Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae

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