FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 137-140.doi: 10.7506/spkx1002-6630-201012031

• Technology Application • Previous Articles     Next Articles

Preparation of Mogroside Ⅴ from the Fruits of Siraitia grosvenorii by Semi-Preparative HPLC

NING De-sheng1，LIANG Xiao-yan1，FANG Hong1，YAO Chong-hui2   

  1. 1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Science, Guilin 541006, China；
    2. Hezhou Institute of Products Quality Supervision and Inspection, Hezhou 542800, China
  • Received:2009-09-15 Revised:2010-03-23 Online:2010-06-15 Published:2010-12-29
  • Contact: NING De-sheng1 E-mail:ndshgxib@sina.com

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Abstract:

Objective: Semi-preparative HPLC was employed to establish a fast, stable and efficient method for preparation of high purity mogroside V. Methods: The crude mogroside V extract from the fruits of Siraitia grosvenorii was decolorized by D101 macroporous resin column chromatography before semi-preparative HPLC separation. Based on the use of Eclipse XDBC18 (9.4mm× 250 mm, 5μm) held at 38 ℃, the chromatographic conditions employed were as follows: mobile phase, a mixture of acetonitrile and water at a flow rate of 4 mL/min in the gradient elution mode, detection wavelength, 210 nm; abd sample load 36 mg. Results: The purity of the product obtained was as high as 99.1%. Conclusion: The developed method, thanks to the benefits of simplicity, rapidity, good stability and obtaining of high purity products, will have a good application prospect .

Key words: Siraitia grosvenorii, mogroside V, semi-preparative HPLC

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