FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12 ): 212-214.doi: 10.7506/spkx1002-6630-201012049

• Analysis & Detection • Previous Articles     Next Articles

Determination of Glucocorticoid Residues in Pork by HPLC-MS/MS

NIU Jin-yang, SHI Hong-xia   

  1. China Meat Research Center, Beijing 100068, China
  • Received:2010-01-26 Online:2010-06-15 Published:2010-12-29

Abstract:

A sensitive and rapid method using HPLC-MS/MS was proposed for the determination of 8 glucocorticoid residues in pork. Samples were extracted with methanol and cleaned up on a solid-phase extraction column before HPLC-MS/MS analysis. Optimum sample pretreatment conditions and mass spectral parameters were investigated. Calibration curves for 8 glucocorticoids showed good linearity over the concentration range from 0.5 to100μg/kg. Average recoveries for them spiked at 3 levels were between 78% and 101%. The method developed exhibited a limit of detection for all the analytes ranging from 0.2 to 2μg/kg. The determination of real samples was carried out using this method and satisfying results were obtained. This method is simple, rapid, sensitive and highly selective and is worth popularizing among food inspection organizations.

Key words: glucocorticoid, pork, HPLC-MS/MS

CLC Number: