FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 215-218.doi: 10.7506/spkx1002-6630-201012050

• Analysis & Detection • Previous Articles     Next Articles

A HPLC Method for the Determination of Sialic Acid in Antler Velvets

LU Xiu-xiu1，LI Gang2,3，XUE Dong-hua1，ZHANG Long2,4,*   

  1. 1. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China；
    2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, China ；
    3. Jilin Province Product Quality Supervision and Inspection Institute, Changchun 130022, China ；
    4. School of Chemical Engineering, Changchun University of Technology, Changchun 130012, China
  • Received:2009-09-15 Revised:2010-02-01 Online:2010-06-15 Published:2010-12-29
  • Contact: ZHANG Long E-mail:zhanglongzhl@163.com

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Abstract:

A cationic exchange-HPLC method was presented for determination of sialic acid in antler velvets. Hydrochloric acid was used to hydrolyze samples. The optimum determination conditions based on the use of ZORBAX 300-SCX cationic exchange column were determined as follows: mobile phase, a mixture of acetonitrile and 0.1% phosphoric acid (90:10, V/V) at a flow rate of 1.0 mL/min; sample loading amount, 10.0 μL; and detection wavelength, 205 nm. There was good linearity between peak area and sialic acid concentration within the range from 5 to 100μg/mL. The detection limit (RSN = 3) of the method developed was 0.03 g/kg for sialic acid. The average recovery for sialic acid was 83.4%, with a RSD of 4.9% (n = 6). This method is applicable for the determination of sialic acid in antler velvets and antler velvet products.

Key words: antler velvet, sialic acid, high performance liquid chromatography, SCX cationic exchange column

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